Advancing Acoustic Ejection Mass Spectrometry: next-generation platform for high-throughput sample analysis

The speed and quality of fluorescence-based high-throughput screening (HTS) methods have become critical problems in the drug discovery process, yet these methods remain common due to their sensitivity, ease of use and adaptability to HTS formats. The use of labeled compounds increases the cost per sample and the detected response can be affected by fluorescent quenchers, colored compounds and form aggregates. These pan-assay interference compound (PAIN) effects are problematic, as they produce false positive results and are difficult to predict.

Alternative approaches to fluorescence assays for high-throughput sample analysis are being implemented, such as mass spectrometry, which utilizes label-free detection of the analyte using the mass-to-charge ratio. However, the traditional LC-MS analysis of 384 samples requires hours, compared to the seconds needed fora plate reader, and typically necessitates different buffer requirements. The Echo® MS+ system with the ZenoTOF 7600 system presents an alternative approach for analyzing small molecules and intact proteins and can solve some of the challenges faced by traditional techniques in biologically relevant buffers.

In this webinar you will learn:

• AEMS technology
• Key features of new Echo® MS+ system
• HT applications (intact proteins)

Presenter: Jacob W. McCabe, Ph.D. (Senior Scientist – Echo MS Excellence Team, SCIEX)

Jacob W. McCabe, Ph.D. is a Senior Scientist at SCIEX in the Echo® MS Center of Excellence in Framingham, MA. The lab is a dynamic and interactive scientific showroom containing new and cutting-edge innovations in mass spectrometry. Jacob and colleagues collaborate with researchers from around the globe to develop new approaches to high throughput quantitative mass spectrometry across a range of molecule types and analytical challenges.He holds a Bachelor of Science degree in chemistry from Baylor University, a Master of Science from Texas A&M University – Commerce, and a Doctor of Philosophy in Analytical Chemistry from Texas A&M University studying under Professor David H. Russell.