5 Rules for Successfully Developing and Scaling Up Your LC Purification

Chemists have been diligent in their pursuit to uncover best practices for achieving more reliable scale up from analysis to preparative liquid chromatography (or PREP LC). PREP LC is the primary technique used to isolate target compound(s) from complex reaction mixtures in support of molecular synthesis. Predictably scaling an LC separation to produce highly pure molecular targets is not trivial and the risk of sample loss is real.

Because chemists spend days and weeks creating their precious new molecular targets, leaving success to chance in scaling an LC separation is ill-advised. Method conditions need to be maintained and care taken to achieve direct scale-up in prep separations. By following just 5 simple rules for LC scale up, the chemist can likely avoid surprising outcomes such as inadequate compound purity (requiring rework) and target recovery. A review of scale-up rules as well as practical guidance for first-time purification success will be the focus of this webinar.

**Key Learning Objectives: **

• Learn to scale your analytical – prep methods predictably.
• Gain knowledge from practical guidance shared for first-time purification success when scaling your LC separation.
• Discover how following the rules of scaling PREP LC simplifies the process
• Learn how to better control LC scale-up outcomes and boost both target purity and recovery.

Presenter: Kathy Lawrence (Sr. Product Marketing Manager, Waters Corporation)

Kathy Lawrence is the Sr. Product Marketing Manager for prep column technologies at Waters Corporation. Early in her career, she was a discovery R&D chemist and a CRO lab manager gaining valuable experience isolating natural products and synthesizing small molecule targets. Later, as Waters North America Purification Specialist, she travelled the continent supporting the success of others purifying targets at various scale. Kathy has held advanced leadership roles in marketing and business development in support of the commercialization of systems, services, and consumables. She holds a Masters degrees in both chemistry and business, is an author of some scientific publication, and is a patented product inventor.