Back to basics: Revealing the strength of HPLC-CAD methods

In recent years, the use of quantitative liquid chromatography (LC) coupled charged aerosol detection (CAD) for poor UV absorbing analytes in multicomponent mixtures has grown exponentially across industrial sectors.

In this live webinar, we will highlight some distinctions between CAD and other UV-absorbance detection, such as the non-linear response to analyte concentration, and sensitivity to mobile phase impurities. However, careful optimization of CAD parameters can lead to successful development and transfer of analytical methods, nearly on par with that of conventional UV absorbance detectors. Through careful tuning CAD parameters, we have developed quantitative LC-CAD assays which are sensitive, precise, accurate and robust across early and late-stage pharmaceutical development. Examples of LC-CAD methods across various stages of drug development spanning from discovery to commercialization will be shared during the presentation.

Learning points:

• Optimization: Optimizing non-linear detection response
• Troubleshooting: Tips and tricks to maintain a CAD detector
• Transfer: Examples of HPLC-CAD successfully developed and transferred (nearly on par with conventional HPLC-UV)
• Implementation of HPLC-MS-CAD for post µg-purification analysis

Who should attend:

• Analytical chemists: Professionals who specialize in analytical techniques, especially those involved in liquid chromatography and detection methods
• Pharmaceutical scientists: Researchers and scientists working in the pharmaceutical industry, particularly those involved in drug development and quality control
• Quality control and assurance professionals: Individuals responsible for ensuring the quality and reliability of analytical methods used in various industries, including pharmaceuticals

Presenter: Imad Haidar Ahmad, PhD (Research Director, Amgen)

Presenter: Wes Barnhart (Principal Scientist, Amgen)